Merck Millipore ProteoExtract Kits

Merck Millipore has launched two ProteoExtract Kits that enable researchers to more rapidly and effectively enrich, isolate and analyse the cytoskeleton and associated proteins.

The ProteoExtract Cytoskeleton Enrichment and Isolation kit (cat. # 17-10195) provides the necessary extraction buffers to quickly and selectively remove soluble cytoplasmic and nuclear protein fractions for downstream biochemical analysis, including Western blot and mass spectrometry. The assay is performed in as little as 20 min and foregoes the need for harsh buffers and centrifugation, thus retaining more of the native focal adhesion- and actin-associated proteins, as compared to traditional protein fractionation methods. Vimentin and GAPDH antibodies are also included as markers for the cytoskeleton and cytosol, respectively, for Western blot analysis.

The ProteoExtract Native Cytoskeleton Enrichment and Staining Kit (cat. # 17-10210) includes the necessary reagents to efficiently enrich, retain and visualise the cytoskeleton and associated proteins in their native, adhered conformations with the same gentle technique. The kit also includes labelled phalloidin and antibodies against vinculin and GAPDH as markers for visualisation and co-localisation of the actin cytoskeleton, focal adhesions and cytosol for immunofluorescence analysis.