Merck Millipore PureGenome Low Input NGS Library Construction Kit

The PureGenome Low Input NGS Library Construction Kit streamlines the process of generating indexed or barcoded libraries for Illumina HiSeq sequencing from low amounts of input DNA (from 50 pg to 1 ng). The kit is suitable for applications such as ChIP-Seq (chromatin immunoprecipitation followed by next-generation sequencing), as well as other sequencing applications where input DNA is limited.

To achieve a high level of sensitivity, the kit utilises technology that adds adapters to sheared genomic DNA with ultrahigh efficiency. This methodology enables the previous limits of NGS library construction to be reduced to below 1 ng. The approach greatly simplifies NGS library construction, enabling the construction of instrument-ready singleplex or multiplex libraries in 2 h.

Compared to conventional approaches that require DNA isolation between protocol steps, the process occurs in a single tube and does not require any in-process DNA isolation steps. Once library construction is complete, DNA purification and size selection is performed using the Agencourt AMPure XP beads provided with the kit.