Mercodia’s Lp(a) ELISA provides a method for the quantitative determination of human lipoprotein(a) in serum or plasma. It is a solid phase two-site enzyme immunoassay based on the sandwich technique, in which two monoclonal antibodies are directed against separate antigenic determinants on the apolipoprotein(a) molecule. Apolipoprotein(a) in the sample reacts with anti-apolipoprotein(a) antibodies bound to microtitration wells and peroxidase-conjugated anti-apolipoprotein(a) antibodies in the solution.
Each ELISA kit contains reagents for 96 wells, allows for a sample volume of 25 µL, has an assay range of 0.3–5.0 U/L with a detection limit of 0.07 U/L, and has a total incubation time of 2 h and 15 min. Plasminogen and apolipoprotein B cross-reactions have not been detected.
Mercodia’s Human Lp(a) ELISA kits are distributed in Australia and New Zealand by Sapphire Bioscience.
Phone: 02 9698 2022
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