Meso Scale Discovery S-PLEX ultrasensitive assay platform

Many biomarkers are readily measurable in the pg/mL to µg/mL range. Some analytes are present at much lower concentrations, making them difficult to detect.

S-PLEX is the latest assay range from Meso Scale Discovery (MSD) to improve sensitivity. The ultrasensitive assay is designed to reduce the lower limit of detection (LLOD) by 10- to 1000-fold over other immunoassays, with detection limits in the low fg/mL range.

The format builds on existing MSD consumables and instruments but uses a different chemistry to the company’s other assay methods. High sensitivity and specificity are achieved with an electrochemiluminescent label, Turbo-TAG, which when combined with a detection antibody conjugated with a Turbo-Boost label results in larger signal generation.

The assay is said to allow shifts in the dynamic range, with the lower detection limits enabling detection of analytes in samples that are not readily detected by other assay formats. Small changes in biomarkers related to disease progression or drug administration can thus be detected.

The assays are available as single-plex kits for over 20 different biomarkers including SARS-CoV-2 N and SARS-CoV-2 spike protein. A multiplex S-PLEX Proinflammatory Panel, including nine biomarkers associated with inflammation response and immune system regulation, is also now available.