Metabolite detection kits

Tuesday, 07 April, 2009 | Supplied by: Sapphire Bioscience


The Cayman Chemical Nitrate/Nitrite Colormetric Assay Kit provides an accurate and convenient method for measurement of total nitrate/nitrite concentration in a simple two-step process.

The first step is the conversion of nitrate to nitrite utilising nitrate reductase. The second step is the addition of the Griess Reagents, which convert nitrite into a deep purple azo compound. Photometric measurement of the absorbance due to this azo chromophore accurately determines nitrite concentration.

The kit, which consists of 2 x 96-well plates, can be used to measure nitrate and nitrite in plasma/serum, urine, tissue culture media and tissue homogenates.

Cayman Chemical also supplies a LDH method kit in a 96-well plate format for analysis of nitrate and nitrite from an in vitro assay of nitric oxide synthase (NOS) in which excess NADPH has been added.

Online: www.sapphirebioscience.com
Phone: 02 9698 2022
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