Neogen ANSR Listeria Right Now detection system

The Neogen ANSR Listeria Right Now system is able to detect low numbers of Listeria spp., including L. monocytogenes, from environmental samples without enrichment. Detecting possible environmental Listeria contamination in 1 h allows food manufacturers the ability to find a potential problem quickly and fix the issue before it becomes a serious problem, ie, detect, clean and retest in the same shift.

The system employs an isothermal, amplified nucleic acid-based reaction to target ribosomal RNA. Amplification occurs through a polymerisation mechanism by a specific endonuclease with a fast cycle time. Detection occurs in real time using a fluorescent molecular beacon.

Ribosomal RNA is present in greater numbers in Listeria cells than the traditional DNA target (~1000–10,000 copies per cell vs 1 copy per cell for DNA). This can result in a 1000–10,000-fold increase in target analyte concentration. The availability of more targets and the fast cycle time enable detection in 1 h without the need for enrichment.