NH DyeAGNOSTICS Smart Protein Layers for analysis of protein gels and western blots

Smart Protein Layers (SPL) technology, from NH DyeAGNOSTICS, is suitable for stain-free, quantitative and standardised analysis of protein gels and western blots. The technology is based on the use of three main components: Smart Labels, Smartalyzer and Calibrator.

Smart Labels are fluorescent dyes which bind covalently to proteins. Smart labelled proteins can be visualised directly after electrophoresis — no staining or destaining steps are required. Smart Labels are highly sensitive with a wide dynamic range.

The Smartalyzer is a bifluorescent standard added to each sample prior to separation. The standard provides precise normalisation and quantification of protein gels and western blots within one experiment.

The Calibrator is a second fluorescent standard which is applied to the gel and allows for quantification of data derived from different experiments. It also works as a fluorescent protein molecular weight ladder.

The system enables simultaneous detection of the amount of target protein and total protein in a gel and also between gels. It allows the user to compare lane-to-lane, gel-to-gel and blot-to-blot.