Nordic-MUbio FIX&PERM Cell Fixation and Permeabilisation Kit

Intracellular staining of protein biomarkers using antibodies in flow cytometry has opened up diverse options for phenotypic and functional characterisation of cells in both research and clinical diagnosis. Indeed, definitive phenotypic identification of certain cell types can require labelling of both cell surface and intracellular markers. Additionally, the cytoplasmic localisation of typical membrane molecules can prove to be a useful lineage marker.

In such cases, and for many other cell biological and functional investigations, intracellular immunostaining is a necessity. For this purpose, Nordic-MUbio offers the FIX&PERM Cell Fixation and Permeabilisation Kit.

The kit offers a simple procedure making use of two reagents. Reagent A gently fixes cells, while Reagent B permeabilises them. The specific formulations reduce background and allow simultaneous addition of permeabilisation medium and fluorochrome labelled antibodies. This allows staining of intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins and more.