Oxford Expression Technologies superSf9 cells

The superSf9 cells from Oxford Expression Technologies offer improved recombinant protein yield using the baculovirus protein expression system. The superSf9 cells are transgenic insect Sf9 cells that have been engineered to stably express an additional protein. The presence of this protein leads to prolonged longevity after infection with baculovirus and up to a 15-fold increase in recombinant protein production in baculovirus infected superSf9 cells compared with standard Sf9 cells.

Three types of cells are available: superSF9-1 for improved expression of stable proteins - this offers a longer expression time that enhances the yield of stable proteins. These cells are suitable for intracellular, membrane and secreted proteins, provided that these proteins are not subject to degradation; superSF9-2 for improved expression of toxic proteins. This features an intense peak of recombinant protein production which makes them suitable for increasing the yield of toxic or unstable proteins; superSF9-3 for improved expression of average proteins. This has a phenotype intermediate between the SF9-1 and SF9-2 cells and is suitable for proteins of mixed or unknown character.