PerkinElmer Opal Multiplex Tissue Staining

Sunday, 01 February, 2015 | Supplied by:

Understanding cancer biology and assessment of complex biological function requires simultaneous interrogation of multiple pathways combined with context provided by tissue images. Standard methods provide an incomplete picture: flow cytometry excels at multiplex analysis but destroys the context provided by tissue, and analysis of individual markers in serial sections is unable to promote understanding of single cell co-expression, particularly for three or more markers at a time.

With Opal Multiplex Tissue Staining, users can overcome these challenges and achieve robust, specific multiplex tissue biomarker measurement. Antibodies can be selected based on performance rather than species. The typical 4-plex Opal staining procedure may be completed within one day. The method is compatible with the standard research IHC workflow in the lab and is amenable to automation.

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