Promega Nano-Glo Dual-Luciferase Reporter Assay

The Nano-Glo Dual-Luciferase Reporter (NanoDLR) Assay, from Promega, is a two-reporter system that incorporates NanoLuc luciferase technology. The product provides good data quality and sensitivity for biologically complex applications.

The assay allows researchers to measure NanoLuc and firefly luciferases together in a convenient, easy-to-use format. Firefly luciferase, NanoLuc luciferase or both can be used as dynamic reporters, increasing versatility by allowing researchers to choose the primary reporter that best meets their experimental needs. Users thus gain the sensitivity to detect small changes in expression, flexibility in assay design and robust control reporter options.

The assay chemistry provides improved reagent stability over time, giving consistent results and eliminating the need to freeze reagent. NanoDLR is a homogenous reagent system with stable glow-type luminescent signals but can also be used with injection-based protocols or cell lysates. It is compatible with automated liquid handling and high-density plates, including 1536-well formats.

One novel application of the two powerful primary reporters in the assay is the coincidence reporter assay - an innovation which is promising for high-throughput chemical library screening in drug discovery. This type of assay uses two independent reporters to measure a single transcription event, providing a way to distinguish true ‘hits’ from experimental artefacts caused by interaction between library compounds and reporter proteins.