Promega has launched its Wizard HMW DNA Extraction Kit for researchers working with large fragments of genomic DNA. The kit enables researchers to obtain DNA that will provide strong performance in long-read sequencing applications in a 90 min manual protocol.
For scientists who are looking at an organism that’s never been sequenced or dealing with structural variations and rearrangements within the genome, it’s difficult to take short reads to come up with a large sequence. A lot of workflows have been designed to purify smaller nucleic acids, but the Wizard HMW DNA Extraction Kit is able to purify a high proportion of fragments over 125 kb. In long-read sequencing, the DNA from the kit produces long maximum reads and high N50 values, which indicates a higher proportion of large fragments in the sample.
The kit supports purification from a variety of sample types including whole blood, bacterial cultures, cultured mammalian cells and plant leaf tissue. It joins an existing portfolio of both manual and automated kits to extract a variety of nucleic acids from a broad range of sample types.
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