Cayman Chemical’s Protein Carbonyl Assay Kit is a colorimetric assay for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine (DNPH) and protein carbonyls.
Formation of a Schiff base produces the corresponding hydrazone, which can be analysed spectrophotometrically at 360-385 nm.
This assay can be used to measure oxidised protein in plasma, serum, cell lysates and tissue homogenates.
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