Protein inhibition characterisation

Wyatt Technology has published an application note demonstrating the advanced capability of Composition Gradient Multi-Angle Light Scattering (CG-MALS) technology.

The experiment assessed the performance of Wyatt Technology’s Calypso automated composition gradient light scattering system for the evaluation of the inhibition efficacy of AEBSF, a small-molecule, irreversible inhibitor, on the self-association of the digestive enzyme &$945;-chymotrypsin. The Calypso is able to perform accurate and rapid analysis of inhibition kinetics of protein-protein binding as well as equilibrium association properties.

The application note, entitled Inhibition Kinetics of Protein-Protein Binding with Calypso, is available to download free of charge via www.wyatt.com.

CG-MALS is a powerful, non-destructive method for efficient characterisation of macromolecular interactions without labelling, immobilisation or additional biochemical substrates. It employs a series of samples of different composition or concentration in order to characterise reversible self- and hetero-association of proteins, reaction rates and affinities of aggregation or dissociation and non-specific, repulsive or attractive macromolecular interactions.

The Calypso’s analytical capabilities simplify and automate CG-MALS measurements. The system’s automation enhances productivity by improving repeatability and reliability, while minimising time and effort.