Proteome analysis

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Spotlighting the flexibility of Shimadzu's Prominence Series in creating specific application systems, this series includes the 2D Micro-HPLC System for proteome analysis, the 2D HPLC for bioanalysis (BA), and the 2D HPLC for NMR sample preparation. All three feature the Shim-pack MAYI Restricted Access Media (RAM) column, a bio-sample pre-treatment column that achieves highly efficient deproteinisation and long-term stability through optimisation of particle diameter and newly developed coating technology.

The high-performance 2D Micro-HPLC System for proteome analysis performs separation of complex peptide mixtures with a high level of sensitivity and resolution, and enables analysis of highly ionic and hydrophobic proteins that cannot be analysed with 2D gel electrophoresis.

Assay sensitivity enables trace peptide analysis not always detected on 2D gels. Analysis fractions from the cation exchange column are trapped on six mini trap columns (polymer packed reversed-phase columns with a retention power equivalent to C4) for desalination, followed by back flushing onto a reversed-phase column for precise and reproducible results.