Quansys Biosciences Q-PLex Singleplex assay

Q-Plex Singleplex assays are single analyte microarrays used to measure a range of human cytokines. The kits adopt high dynamic range (HDR) technology to measure a significantly greater quantifiable range compared to traditional ELISAs.

HDR technology involves the use of antibodies optimised for high- and low-range measurement of specific analytes. The antibodies are printed in planar arrays at the bottom of each microplate well, along with a reference spot for convenient imaging.

QView Software combines the data from all the microspots using a proprietary algorithm based on five-parameter logistic regression modelling. This ensures quality analyte readings with maximum low-end sensitivity and peak high-end quantification. Kits are efficient and easy to run, requiring only 50 µL per sample and using a protocol similar to traditional ELISAs.

The expanded range across the standard curve means fewer dilutions per sample; better sensitivity than traditional ELISAs to capture low analyte levels; and the higher end range captures data for concentrated samples. The intra-well precision from replicate microspots also provides greater surety of results.