Rapid, high-yield protein production

Friday, 08 June, 2007 | Supplied by: http://www.invitrogen.com/

Rapid, high-yield protein production

The FreeStyle MAX System is a breakthrough technology for rapid and high-yield mammalian protein production.

This new mammalian expression technology is available in two convenient formats. FreeStyle MAX Reagent is a novel cationic lipid-based reagent designed to transfect FreeStyle cells with high efficiency. The FreeStyle MAX Expression System combines GIBCO FreeStyle Media, FreeStylâ„¢ MAX Reagent, and either FreeStyle CHO-S cells or FreeStyle 293 cells. The only equipment required to use the FreeStyle MAX System is a CO2 incubator and a platform shaker with shake flasks or a spinner culture.

The FreeStyle MAX System provides:

  • An easy, rapid protocol for transient transfection of CHO and 293 cells in suspension cultures
  • Functional proteins (up to milligram quantities) with all post-translational modifications produced in one week
  • Simplified downstream purification of secreted proteins with FreeStyle serum-free medium
  • Straightforward protocol for production scale-up
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