Sartorius Stedim Biotech ambr 15 cell culture Generation 2

Sartorius Stedim Biotech’s (SSB) ambr 15 cell culture Generation 2 is the successor to the ambr 15 cell culture system, shown in the industry to reduce cell line development timelines and increase lab productivity. The latest version offers increased flexibility and expanded capability for clone selection, media and feed optimisation and early process development work.

The product replicates laboratory-scale bioreactor performance at the microscale (10–15 mL) and controls up to 48 single-use bioreactor cultures in parallel. Its updated design incorporates novel features to provide greater process flexibility, expand functionality and allow more applications to be investigated. In addition, ambr clone selection software, included as a one-year licence, provides simplified, streamlined multivariate data analysis for fast, consistent cell line screening and ranking.

Selection of clones, media and feeds can be performed under perfusion mimic conditions to bleed large volumes of culture and quickly remove spent media from the microbioreactor. The culture station design provides low stirrer speed control suitable for sensitive cell lines. A flexible workstation layout and an expanded tip bin capacity provide operator walkaway time.

Adaptation of cell lines to different media for media screening studies can be performed in the microbioreactor using culture passage steps and rapid vessel drain functionality. Media mixing steps automate the creation of media blends, eliminating the need to pre-mix, saving time and reducing chance of error. Clone stability studies can also be set up with serial passages performed and fully automated in the microbioreactors.

The system provides a robust screening platform for development of cell and gene therapy processes, including HEK293 for viral vector production, T-cells, iPSCs and other immune-derived cell lines. Cell and gene therapy applications are supported by design features that include rapid vessel drain functionality for automated cell passaging and media exchanges in the microbioreactors.