Shimadzu PPSQ series protein sequencer

Friday, 25 November, 2011 | Supplied by: Shimadzu Scientific Instruments (Oceania) Pty Ltd

Providing a simple and robust solution for many laboratories, protein sequencing utilising automated Edman degradation chemistry remains a vital method of producing unambiguous data on biopharmaceutical or biochemical materials. An Edman sequencer, such as Shimadzu’s PPSQ series instrument, is suitable for producing N-terminal sequencing information for proteins and peptides and is less complex to use than many alternative mass spectrometry-based techniques.

Shimadzu’s PPSQ series achieves greater simplicity and reliability in the automatic determination of amino acid sequences by employing HPLC analysis coupled with high sensitivity detection and powerful acquisition/analysis software. N-terminal sequencing is a key part of the ICH Q6B guidelines for characterisation and confirmation of biopharmaceuticals. The PPSQ Series has been specifically developed to simplify the generation of customised reports for information relating to estimated sequences and yield rates which can easily be displayed in graphical form to enable users to select amino acids for their calculations.

Suitable for supporting QA applications in biopharmaceutical and biochemistry companies, the Shimadzu PPSQ series is also suitable for university departments looking to run protein sequencing studies.

The PPSQ series’ software features control functions for the reaction unit and HPLC, enabling easier sequence analysis of proteins and peptides. Easy-to-use data analysis functions simplify the reprocessing of chromatograms as well as the overlaying of multiple chromatograms which all helps in the automatic estimation of amino acid sequences.

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