Sigma Life Science Onyx Quencher for qPCR

A quencher for quantitative real-time PCR (qPCR) that improves assay signal quality, Onyx Quencher adds to Sigma Life Science’s portfolio of qPCR probes, reagents and oligonucleotide services.

The product is a dark quencher and therefore produces heat instead of light on excitation. This is said to improve the signal-to-noise ratio over traditional fluorescent quenchers, such as TAMRA.

Available with the company’s Custom Primers and Dual-Labeled Probes, four derivative versions of Onyx Quencher extinguish light over an excitation maximum range from 515 to 661 nm, which includes the emission spectrum of common reporter dyes such as 6-FAM. Performance of all four versions has been validated with several reporter dyes, with all performing equivalently to a comparable dark quencher.

In addition to qPCR, the product may be used for other applications and is a suitable quencher for the development of life science research tools, molecular diagnostics (MDx) and laboratory developed tests (LDTs).