Sphere Fluidics Cyto-Mine single-cell analysis system for cell line development

Sphere Fluidics’ Cyto-Mine is a single-cell analysis platform that enables individual cells to be encapsulated within picolitre-sized aqueous droplets in a biocompatible carrier oil (picodroplets). Each picodroplet provides a controlled, defined and adjustable environment that maintains cell viability. Single cells may be screened with speed and precision while secreted proteins are trapped within the picodroplets, making them easily accessible for characterisation.

Multiple procedures and assays within the cell line development pathway can be miniaturised and integrated within the platform, allowing tasks that would previously have taken weeks to complete to be undertaken within one day. Approximately 200,000 individual cells can be screened in a matter of hours using this approach, compared with around 10,000 cells with manual analysis techniques. Quality and quantity of molecular product (monoclonal antibody) are also enhanced as screening can be conducted at an early stage, so that only cells expressing the protein of interest, and the best-performing cells within this pool, are taken forward in the development process.

Single cells are visualised using the specially designed Cyto-Mine picodroplet imaging technology and monoclonality data can be provided to support regulatory submissions. The complete analytical process is biocompatible and low-stress for cells. The platform is designed using integrated, user-friendly software that requires minimal specialist training to understand and implement effectively.