The iTaq fast supermixes with ROX can be used on a variety of instruments, including ROD-dependent real-time PCR systems, and are designed to deliver qPCR efficiency, sensitivity and specificity, with a robust fluorescent signal under fast or standard cycling conditions with real-time detection chemistry.
The supermixes are preblended with hot-start iTaq DNA polymerase, buffer, nucleotides and ROX passive reference dye. The one-tube formulations correct inter-well signal variation.
The system is suitable for gene expression analysis. Simultaneous detection of two gene targets using fluorogenic probes can be performed with no observed differences for detection of low-expressing genes in singleplex or duplex.
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