Tecan Rapid EZ DNA-Seq library preparation kit

Tecan’s Rapid EZ DNA-Seq library preparation kit is designed to offer PCR-free generation of sequencing-ready libraries for next-generation sequencing (NGS). Suitable for whole genome sequencing and metagenomics for samples with varied GC content, as well as amplicon sequencing, the automation-friendly kit complements Tecan’s existing Celero, Rapid and Ovation Ultralow DNA-Seq library preparation solutions.

The library preparation kit is an end-to-end solution for PCR-free generation of NGS libraries in 2.5 h. The kit uses Tecan’s robust enzymatic DNA fragmentation and DimerFree library construction technologies, eliminating the need for tedious optimisation regardless of input amount. It offers an ‘add and incubate’ workflow, making it suited to applications sensitive to PCR bias or PCR artefacts, such as microbiome and prokaryotic samples, or sequencing of GC- or AT-rich regions of the human genome.

The kit includes all of the reagents needed for library construction, with pre-plated Unique Dual Index (UDI) adaptors to enable flexible multiplexing and detection of ‘index hopping’. The simple, flexible workflow can be easily automated on Tecan’s liquid handling platforms, such as the DreamPrep NGS workstation.