Gram stain technical update
BD Diagnostic Systems has developed and incorporated a filtration process improvement to reduce the presence of artifacts when using Difco-BBL Gram Stains.
Safranin counterstain contains varying amounts of insoluble, spherical, chemical precipitate that could be mistaken for gram-positive cocci in Gram stain preparations. The spheres are present in low numbers, from 0 to 10 per 20 fields examined, depending on the source of the powdered stain and the methods used to prepare the stain. The spheres are usually single, rarely in pairs, but never appear in clusters or chains. With the implementation of the filtration step in the manufacturing process for safranin counterstain, the frequency of artifacts has been significantly reduced resulting in Gram stain preparations that are clearer and easier to read.
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