Maldi-mass spectrometry matrices

Thursday, 20 February, 2003 | Supplied by: Merck



Maldi (matrix-assisted laser desorption/ionisation), a laser-based soft ionisation method, is one of the most successful ionisation methods for mass spectrometric analysis and investigation of large molecules. In addition, analysis of post source decay (decay of proteins following the ionisation) in many cases allows rapid sequencing of proteins. Thus Maldi has gained a crucial importance for protein analysis.

Its constituting feature is that the sample is embedded in a chemical matrix (ca 1000 x molar excess) that greatly facilitates the production of intact gas-phase ions from large, non-volatile and thermally labile compounds such as proteins, oligonucleotides, synthetic polymers, and large inorganic compounds. The matrix plays a key role in this technique by absorbing the laser light energy and causing a small part of the target substrate to vaporise.

Fluka provides Maldi-Mass Spectrometry matrices applicable to a wide range of applications including peptide/protein, oligonucleotide and carbohydrate analysis. Additional information is available in the complimentary newsletter AnalytiX 6/2001 Maldi-Mass Spectrometry.

Online: www.sigmaaldrich.com
Phone: 02 9841 0555
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