Mammalian Genomic DNA Purification Kit
The Sigma GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure high molecular weight DNA from a variety of mammalian sources.
This kit uses a silica-based membrane specially selected for genomic DNA purification in a convenient spin column format. Cells and tissues are lysed with a chaotropic salt-containing buffer to ensure denaturation of macromolecules. The addition of ethanol causes DNA to bind to the membrane and the remaining lysate is removed by centrifugation. After washing to remove contaminants, the DNA is eluted into a collection tube.
The purified DNA may be used in many applications such as sequencing, cloning, blotting, restriction enzyme digestion, ligation and PCR.
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