Qiagen has added to its portfolio of miRNA technologies with the introduction of the miScript System, which enables detection and quantification of miRNA, as well as mRNA and other small RNA, using SYBR Green-based, real-time PCR.
The miScript System allows quantification of multiple miRNAs from a single cDNA synthesis reaction, reducing variability and saving samples. Both miRNA and mRNA can be quantified from the same cDNA synthesis reaction, enabling simultaneous detection of reference genes or other mRNAs of interest, such as the mRNA targeted by an miRNA.
The miScript System is highly specific, overcoming the challenges presented by the existence of multiple miRNA isoforms. In addition, high sensitivity provides a dynamic range claimed to be several orders of magnitude greater than microarrays, ensuring comprehensive and accurate miRNA profiles.
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