Microarray fluorescence reader

Monday, 18 August, 2003 | Supplied by: Beckman Coulter Australia



Beckman Coulter has introduced a fluorescence-based CCD microplate reader for the automatic quantitation of hundreds of protein analytes on the A2 MicroArray Plate. The A2 MicroArray Reader has been specially designed to work with the A2 MicroArray System to quantitate multiplexed immunoassays. Part of the ProteomeLab family of products, this protein microarray system has the potential to allow users to detect and quantitate protein-protein interactions, protein immunoassays, DNA-protein interactions, enzymatic reactions and other protein assays.

The A2 MicroArray platform overcomes many of the limitations associated with other microarray systems that involve complicated image processing manipulations. These steps can compromise accuracy and severely limit throughput. In addition, the A2 Reader can read a plate and process the microarray data in less than five minutes, compared to as much as an hour for other microarray systems.

Built-in, easy-to-use, A2 Reader software quickly precisely aligns a grid around each of the spots on the A2 MicroArray Plate and measures the fluorescent intensity, which can then be correlated to analyte concentration. The A2 Reader utilises CCD camera technology to image one well at a time and up to two fluorescent wavelengths per scan.

The A2 Reader takes up minimal laboratory space and can be integrated with the Biomek automated liquid handling platforms for complete automation of the assay. With this approach of creating an array within a microtiter well the sample preparation process as well as the detection and measurement of the analytes can be automated with little or no manual intervention.

ProteomeLab is a family of proteomic tools for cellular identification and protein isolation, fractionation, characterisation and evaluation. These tools can ultimately be used to identify markers for the development of disease diagnostics and therapeutic targets.

Online: www.beckman.com.au
Phone: 1800 060 878
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