Protein blotting handbook

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Millipore has released the fifth edition of its Protein Blotting Handbook. The publication includes techniques for protein blotting including 17 protocols for protein transfer, visualisation and immunodetection.

The handbook also contains a detailed troubleshooting guide and several innovative methods, such as the Rapid Immunodetection Method that reduces detection time by several hours and the Double Blot Method that eliminates non-specific binding.

Protein blotting is a key technology used by every protein laboratory to detect low amounts of proteins in complex samples or monitor protein expression and purification.

The handbook's membrane selection guide compares and contrasts the two most commonly used membranes for western blotting applications. It also defines the basic principles and methods for process optimisation.

The protocols section is a compendium of both traditional blotting detection methods, such as dot, slot and western blots, and other specialised procedures.