Quantitative PCR

Friday, 14 May, 2004 | Supplied by: http://www.sigma-aldrich.com/



SYBR Green JumpStart Taq ReadyMix is available in both standard and capillary formulations. It combines the advantage of a hot start enzyme with a ready-to-use mix of SYBR Green 1, dNTPs and optimised buffer solutions.

SYBR Green JumpStart Taq ReadyMix is recommended for single product real-time amplification experiments. It can also be used for PCR optimisation before manufacture of labelled probes. SYBR Green Taq ReadyMix Capillary formulation is a solution for performing quantitative PCR in a capillary cycler. The RT-PCR formulation combines DuraScript Reverse Transcriptase, JumpStart Taq DNA polymerase and SYBR Green in a one step RT-PCR kit designed for measurement of gene expression.

SYBR Green 1 is a fluorescent dye that binds selectively to double stranded DNA. Detection of the DNA can then be monitored by measuring the increased fluorescence throughout the PCR cycles. SYBR Green 1 is sensitive, detecting down to 10 copies of template. The hot-start mechanism using the JumpStart Taq antibody prevents non-specific product formulation.

All formulations are packed as a ReadyMix providing consistent batch-to-batch and reaction-to-reaction performance.

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