Tomocube HT-X1 Plus holotomography and spinning disk confocal microscopy system

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The HT-X1 Plus holotomography (HT) system from Tomocube has been integrated with the X-Light V2 spinning disk confocal microscope (SDCM) from CrestOptics to offer spinning-disk confocal holotomographic imaging. This next-generation multimodal imaging platform, now available from Tomocube, provides high resolution and sensitivity for 3D biophysical imaging applications where 3D holotomography is combined with fluorescence microscopy to allow researchers to correlate quantitative, label-free imaging with molecular-functional information, which should unlock a more advanced understanding of biological processes.

Holotomographic imaging involves illumination of a live cell with low-power visible light from multiple illumination angles, followed by measurement of the phase delay of the transmitted light. The technique uses the measured refractive index as imaging contrast, allowing visualisation of 3D living cells and tissues without labelling or staining. With its high spatial resolution capable of capturing subcellular organelles, users have increasingly sought confocal fluorescence capabilities to design multiplex imaging workflows that minimise photodamage. The HT-X1 Plus combines holotomographic imaging with CrestOptics’ spinning disk high-speed confocal technology within a fully integrated system, in order to enable the acquisition of label-free, high-resolution 3D holotomography data and highly sensitive, precise 3D fluorescence data.

By combining these two imaging modalities, the HT-X1 Plus helps to facilitate deeper, more complete insights into molecular distribution within samples, without requiring invasive methods. Additionally, the integrated platform is designed to provide precision alignment of HT and fluorescence-based imaging datasets, allowing researchers to easily study molecular and phenotypic data in tandem and generate more powerful insights across biophysical imaging applications.