Crest Optics DeepSIM super-resolution confocal microscope module

Friday, 29 October, 2021 | Supplied by: SciTech Pty Ltd


Crest Optics introduces the DeepSIM super-resolution confocal microscope module, designed to enhance the imaging capabilities of confocal microscope systems and compatible with any existing upright or inverted microscope with a camera port. The module can be used with Crest Optics’ X-light V3 spinning disk, or alternative confocal systems, to offer high-resolution images for life science researchers.

Based on a multispot structured illumination system, the easy-to-use module can be used to study cellular structures up to an XY resolution of ~100 nm. Super-resolved optical sectioning, with Z resolution up to ~300 nm, can be obtained using both high (60–100x) and low (20–40x) magnification objectives to expand the range of applications to include complex 3D models such as tissues, organoids, spheroids and small organisms.

The product is designed to work with samples of thicknesses comparable to those used in confocal microscopy, giving super-resolved data over 50 µm Z in depth in non-clarified samples. This means that more meaningful data should be obtainable from native heterogeneous complex samples using routine preparation protocols.

The device enables the study of live-cell dynamics through a temporal resolution greater than 10 fps (1024 x 1024 px FOV), allowing biological changes to be tracked at cellular and subcellular levels. High performance is combined with the flexibility of a modular, expandable system, available either as a super-resolution add-on module for the CrestOptics X-light V3 spinning disk system or standalone equipment.

Online: www.scitech.com.au
Phone: 03 9480 4999
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