Abcam InstantBlue Coomassie Protein Stain

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Abcam’s InstantBlue (ab119211) is a ready-to-use Coomassie protein stain for polyacrylamide gels. Its mechanism of action stains proteins in 15 min while leaving a clear background, eliminating the need to fix, wash or destain.

Formulated for safe use and easy disposal, it is ready to use straight out of the bottle and comes in convenient premixed 1 L volumes. The patented mechanism for rapid Coomassie blue staining of protein gels begins in moments, and results are achieved within 15 min. Some premade and traditional homemade Coomassie R-250 protein stains can take 3 h or more to fully stain gels.

The product means there is no longer any need to wash, fix, microwave or destain gels. Homemade stains often require all these steps and most premade solutions require at least one. InstantBlue uses a simple and easy-to-use, one-step protocol. High sensitivity allows protein levels as low as 5 ng per band to be detected (BSA) when staining overnight.

Key benefits include ultrafast staining in 15 min or less; a one-step procedure with no washing, fixing, microwaving or destaining; high sensitivity with 5 ng bands detectable; clear background with a high signal-to-noise ratio; flexible with no overstaining; efficiency at only 25 mL per mini gel; safe nontoxic composition with no fume hood or solvent disposal required. It is also methanol-free for no gel shrinkage or protein methylation; acetic acid-free for no protein acetylation; mass spectrometry compatible; and destainable with no residual methylation or acetylation.

For more information: https://www.abcam.com/instantbluereg-coomassie-protein-stain-ab119211.html.