Cosmo Bio PUREfrex cell-free protein synthesis reagent

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Cosmo Bio’s PUREfrex kit is a reconstituted cell-free protein synthesis reagent based on PURE system technology. The reaction system consists of proteins, ribosomes, amino acids and NTPs. The proteins are necessary for transcription, translation and energy regeneration. The proteins and ribosomes are highly purified individually and assembled together to constitute the protein synthesis system.

To synthesise a protein, just add the template DNA or mRNA encoding the protein of interest into the reaction mixture and incubate for several hours. The reconstituted system works by assembling translation-related factors only. This means the user may adjust the composition of the reaction mixture as they like.

The components of the kit are said to have improved levels of purity. Cosmo Bio has also reviewed the construct of each component for better activity, so all components of the kit have no tags for purification and detection. This enables fusion of the user’s protein with any tag.

The system is suitable for the preparation of prokaryotic protein, eukaryotic protein, membrane protein, protein containing disulfide bonds, protein containing unnatural amino acids, etc. Other applications include basic research in protein science (translation, folding of protein after synthesis) and in vitro display (ribosome display, mRNA display).