AdipoGen PLXDC2 (human) ELISA Kit

Haematopoietic stem cells (HSCs) are defined by the capabilities of multi-lineage differentiation and long-term self-renewal, allowing the restoration of haematopoiesis after insults, such as infections or therapeutic ablation. Different markers (multi-antibody combination) are used for positive and negative selection (CD34+CD38-CD90+CD45RA-CD49f+) as a conventional protocol to isolate a population of cells that results in only ~10% functional HSCs when human cord blood is used as the starting material.

Recently described in the journal Communications Biology, PLXDC2 is highly expressed in human and mouse HSCs derived from both cord blood and bone marrow, and PLXDC2 can be used as a new key marker to enrich both human and mouse HSCs using flow cytometry. The use of both PLXDC2 and CD150 membrane proteins is sufficient for the isolation of functional HSCs without any additional markers. PLXDC2 in combination with CD150 is designed to be a new, very efficient and easy protocol to highly purify mouse and human functional HSCs compared with the conventional multi-antibody combination.

AdipoGen Life Sciences’ PLXDC2 (human) ELISA Kit measures human PLXDC2 with a sensitivity of 60 pg/mL and range of 0.078–5 ng/mL, using either cell culture supernatant, plasma or serum. AdipoGen Life Sciences provides human and mouse PLXDC2 monoclonal antibodies for flow cytometry, as well as proteins that can be used to study this new role of PLXDC2.

AdipoGen Life Sciences’ PLXDC2 (human) ELISA Kit is distributed in Australia and New Zealand by Sapphire Bioscience.