Enzo Life Sciences Fluor de Lys-Green substrate
The Fluor de Lys-Green HDAC assay is an HTS-compatible, homogenous assay for measuring histone deacetylase (HDAC) or sirtuin activity from cell or nuclear extracts, immunoprecipitates or purified enzymes.
The assay employs Enzo Life Sciences Fluor de Lys-Green, a new substrate claiming higher sensitivity and an excitation and emission (485/530 nm) that avoids quenching and fluorescent interference from compounds absorbing in the near UV and blue range.
The increased sensitivity and longer wavelength excitation/emission avoid interference from auto-fluorescence of cell constituents and test compounds. It is an HTS-compatible, two-step homogenous assay.
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