EpigenTek EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit

N6-methyl-adenosine (m6A) is the most common and abundant modification on RNA molecules present in eukaryotes; it accounts for more than 80% of all RNA base methylations and exists in various species. m6A is mainly distributed in mRNA and also occurs in non-coding RNA, such as tRNA, rRNA and snRNA.

The relative abundance of m6A in mRNA transcripts has been shown to affect RNA metabolism processes such as splicing, nuclear export, translation ability and stability, and RNA transcription. Abnormal m6A methylation levels induced by defects in m6A RNA methylase and demethylase could lead to dysfunction of RNA and cause disease.

The EpiQuik CUT&RUN m6A RNA Enrichment (MeRIP) Kit is a complete set of optimised buffers and reagents designed to enrich an RNA fragment containing m6A from low-input RNA, and to identify region-specific m6A by PCR or profile epitranscriptome-wide m6A by next-generation sequencing using Illumina platforms or other methods. The innovative working principle, optimised protocol, and components of the kit allow for the capture of the m6A fragment with minimal non-specific background levels. The enriched RNA is specifically suitable to construct both non-barcoded (singleplexed) and barcoded (multiplexed) libraries quickly, allowing m6A regions to be mapped with low bias and at a high resolution.

The kit is distributed in Australia and New Zealand by Sapphire Bioscience.