GeneCopoeia GLuc-ON Promoter Reporter Clones

Click2Contact

Using a naturally secreted and robust Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON promoter clones are designed to detect the real-time activities of over 20,000 human promoters using live cell assays. GLuc is 1000-fold more sensitive than firefly or Renilla luciferase.

Each transfection-ready, sequenced-verified promoter clone contains a 1.2-1.5 kb insert, corresponding to the 5′-flanking sequence located approximately 1.5 kb upstream and up to 100 bp downstream of the transcription start site (TSS) of a specific human gene. This insert is placed upstream of the GLuc reporter gene. Since the putative cis-acting enhancer elements are expected to exist in the cloned promoter region, the luciferase activity observed during the reporter assay closely resembles the actual promoter regulation of these genes within human cells.

The dual secreted reporter system using GLuc and SEAP enables transfection normalisation for accurate across-sample comparison.