Malvern NanoSight and Zetasizer for particle characterisation

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Exosome research can be constrained by a lack of suitable methods for characterisation as they fall below the detection range of many currently used techniques. Existing techniques such as electron microscopy offer high-resolution images; however, they usually require time-consuming preparation and analysis, which could potentially damage the sample.

Nanoparticle tracking analysis (NTA) coupled with dynamic light scattering (DLS) offers an approach that is fast, easy to use and accurate. The Malvern NanoSight with NTA allows specific bionanoparticles in liquid suspension to be directly and individually visualised and counted in real time. Particle-size distribution profiles in the range of 10-2000 nm (sample dependent) and concentration measurements are determined simultaneously. Operation in fluorescence mode enables characterisation and differentiation of suitably labelled particles using a range of excitation wavelengths.

The Malvern Zetasizer series uses DLS to extend the measurement range to below 1 nm. The product also enables the measurement of zeta potential, a measure of the magnitude of repulsion or attraction between particles which affects stability and interactions. By combining size distribution, zeta potential DLS measurements and the capabilities of NTA, scientists are able to get a more complete picture of nano processes taking place, including aggregation.