Enzo Life Sciences PCR Decontamination Kit and gDNA Removal Kit

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Contaminating DNA can be a problem when small amounts of target are being detected in a PCR reaction. This can lead to decreased PCR assay sensitivity and false positives. Similarly, in reverse transcription, contaminating DNA can potentially result in inaccurate mRNA quantification when starting with low-copy transcripts or small samples. Enzo Life Sciences offers two solutions to eliminate DNA contamination from PCR mastermixes and RNA preps.

The PCR Decontamination Kit removes DNA contamination from PCR mastermixes. Contaminating DNA can be removed in the presence of primers and probe by utilising a specific dsDNase. The dsDNase is irreversibly inactivated by heating, leaving the mastermix components unaffected. The product reduces background to improve target detection with no effect on PCR sensitivity. It has an easy protocol to decontaminate directly in the mastermix.

The gDNA Removal Kit removes DNA contamination from RNA prior to reverse transcription. It uses a heat labile dsDNase which is irreversibly inactivated at low temperatures. This enables an inactivation step which is gentle enough to preserve both quality and quantity of all present RNA. The product removes gDNA from RNA prior to reverse transcription in the same tube. HL-dsDNase is inactivated without reducing quality or quantity of RNA. The method minimises pipetting steps and is suitable for high-throughput experiments.