GeneCopoeia ExProfile Pathway Gene qPCR Arrays

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ExProfile Pathway Gene qPCR Arrays from GeneCopoeia are easy-to-use tools to study pathway- or disease-related gene expression profiling. All primers are experimentally validated and optimised in a real-time PCR condition.

The arrays are designed for profiling the expressions of pre-made or customised sets of coding genes in various tissues or cells. The resulting differential expressions of profiled genes help researchers to identify those that are biologically significant and relevant to their research. In each 96-well plate, there are up to 84 pairs of qPCR primers and 12 wells of controls which are used to monitor the efficiency of the entire experimental process - from reverse transcription to qPCR reaction.

Each pair of primers used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted mRNA. A cDNA pool, containing reverse transcript products from total RNA of 10 different tissues, was used as the qPCR validation template.

A universal real-time PCR condition was developed for easy profiling and analysis of the gene expression in a high-throughput fashion. The All-in-One First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended and supported RT-PCR reagents for use with the ExProfile gene qPCR arrays. The reagents have been optimised to produce high sensitivity, efficiency and specificity.