LifeSensors Tandem Ubiquitin Binding Entity (TUBEs)
Tandem Ubiquitin Binding Entities (TUBEs) are high-affinity ‘ubiquitin traps’ that bind polyubiquitin chains. LifeSensors has developed TUBE technology for the detection, characterisation and isolation of polyubiquitylated proteins from cells and tissue extracts.
TUBEs have 100- to 1000-fold higher affinity for polyubiquitin chains compared to monomer ubiquitin binding domains (UBDs). They both stabilise and bind to ubiquitylated proteins, serving as a suitable tool for ubiquitologists.
The versatile collection includes reagents for isolation/pulldown of polyubiquitin conjugates (Agarose-TUBEs, GST- and His6-TUBEs). For immunohistochemistry, use Fluorescent TUBEs conjugated to either fluorescein or TAMRA. Use Biotin-TUBEs for the detection of polyubiquitylated proteins by ligand blotting.
Biotin-K63 TUBE 1 displays a high sensitivity and specificity for polyubiquitin chains linked through K63. It is said to be more sensitive and cost-effective for the detection of K63-linked polyubiquitylated proteins compared to a traditional Western blot using chain-specific antibodies. It is suitable for ligand blotting, affinity purification from lysates and biological fluids, and in situ detection.
Other claimed features of the TUBEs include: high binding capacity for pull-downs; higher specificity than antibodies and UBAs; lower cost per experiment.
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