Integrated DNA Technologies PrimeTime Pre-designed qPCR Assays

PrimeTime Pre-designed qPCR Assays work for human, mouse and rat transcriptomes. Users can search and select from the online database that contains thousands of assays. All assays are designed to span exons, protect against non-specific amplifications, avoid known SNPs and minimise the impact of secondary structure. Consisting of two primers and a hydrolysis probe, all three components are shipped in a single tube and are claimed to provide >90% efficiency.

IDT’s flexibility allows users to select from five different dye/quencher combinations and choose a pre-mixed primer to probe ratio from 1:1 to 4:1, thereby maximising multiplexing capabilities. The design process uses current, clean and complete target sequence information to generate accurate Tm predictions and protect against off-target amplifications.

Each oligo undergoes 100% QC by mass spectrometry and is shipped within 2-4 working days.