Protein and peptide analysis

Friday, 08 June, 2007 | Supplied by: Thermo Fisher Scientific

The Thermo Fisher Scientific LTQ XL linear ion trap mass spectrometer is equipped with electron transfer dissociation (ETD).

This ion fragmentation technology provides new peptide structure information not available from conventional dissociation methods.

The addition of ETD to the LTQ XL linear ion trap mass spectrometer provides advantages for protein/peptide characterisation, post-translational modification (PTM) analysis and top-down or middle-down sequencing of proteins and peptides.

ETD technology takes advantage of the ion storage capacity and fast cycle times of Thermo Scientific linear ion trap technology, allowing researchers to conduct rapid, alternating collisionally induced dissociation (CID) and ETD scanning throughout the LC/MSn analysis of complex samples, in a way that is not possible on three-dimensional ion traps. A new version of BioWorks software streamlines the processing of ETD data. CID and ETD spectra are searched independently, with results from both searches combined for easy comparison and sorting.

The combination of LTQ XL and ETD technology produces sensitive, stable, robust ETD spectra using reagent anions generated in a Cl source mounted at the back of the linear ion trap.

ETD produces c- and z-type peptide fragment ions, complementing the y- and b-type fragments generated during traditional CID of peptides by ion trap analysis. This additional information allows for more complete characterisation of peptides and proteins than is possible with CID alone.

In addition, ETD technology is a powerful tool for the analysis of post-translational modifications - ETD fragmentation leaves these modifications intact, rather than causing neutral loss events commonly seen with CID analysis of phosphopeptides.

ETD is just one type of useful ion-ion reaction that can be performed; the ETD source option hardware supports multiple ion-ion reaction types, including proton transfer reaction (PTR).

Phone: 1300 735 292
Related Products

Seegene STARlet-AIOS automated PCR system

Seegene's STARlet-AIOS (All-in-One System) is designed to provide hands-free PCR workflows,...

Dojindo Cell Counting Kit-8 (CCK-8)

The Cell Counting Kit-8 (CCK-8) from Dojindo is a pre-mixed, nonradioactive one-bottle solution...

Cayman Chemical Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit

Cayman Chemical's Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit provides a...

  • All content Copyright © 2022 Westwick-Farrow Pty Ltd