Qiagen Investigator Quantiplex Kit

The Investigator Quantiplex Kit has been designed to quickly confirm that forensic DNA samples contain sufficient DNA for genetic fingerprinting analysis. The kit quantifies the human DNA in the sample and establishes whether it contains inhibitors that may interfere with downstream applications.

DNA quantification with the kit is based on real-time PCR. It provides accurate data in less than 50 min with the Rotor-Gene Q thermocycler but is also compatible with other instruments.

Reliable results are ensured by employing the appropriate target sequence and an integrated internal control that tests for successful amplification or PCR inhibition. The overall sensitivity is less than 1 pg/µL. The procedure is also automateable on the QIAgility system for rapid, high-precision automated PCR set-up.

The kit provides the quickest results and enables a higher accuracy than is found with total DNA quantification. External scientific partners validated the marker for the real-time PCR. No copy-number variation has been detected between genders or across populations, making this marker an ideal target for DNA quantification.

The assay completes the forensic workflow offering at Qiagen from sample extraction solutions optimised for forensic samples, as well as advanced assay set-up and detection platforms tailored to different throughput and application needs in the laboratory.