ROS/RNS detection kit

Enzo Life Sciences’ ROS/RNS Detection Kit is designed to directly monitor real-time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy. The kit includes three fluorescent dye reagents: nitric oxide detection reagent (red fluorescent), oxidative stress detection reagent (green fluorescent) for total ROS detection, and superoxide detection reagent (orange fluorescent).

It also includes the positive controls pyocyanin and L-arginine, which are common inducers of ROS and NO production, respectively, as well as the negative controls, N-acetyl-L-cysteine and c-PTIO (common scavengers of ROS and NO, respectively). Through the combination of the three detection probes with a set of specific inhibitors and activators, this kit enables discrimination among superoxide, nitric oxide and peroxynitrite.