GSH/GSSG assay

Oxford Biomedical Research has released a new assay for GSH/GSSH which is available in two formats - 96 well microplate assay and cuvette assay. Reduced glutathione (GSH) is a tripeptide that contains a free thiol group. GSH is a major tissue antioxidant that provides reducing equivalents for glutathione peroxidase (GPx) catalysed reduction of lipid hydroperoxides to their corresponding alcohols and hydrogen peroxide to water. In the GPx catalysed reaction, the formation of a disulfide bond between two GSH molecules gives rise to oxidized glutathione (GSSG). The enzyme glutathione reductase (GR) recycles GSSG to GSH with the simultaneous oxidation of _-nicotinamide adenine dinucleotide phosphate (_-NADPH2). When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease. Therefore, the determination of the GSH/GSSG ratio and the quantification of GSSG are useful indicators of oxidative stress in cells and tissues.

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