Stomach stem cells switch function upon injury

Friday, 15 July, 2022

Stomach stem cells switch function upon injury

A stomach adult stem cell population can fulfil two distinct functions: either help with digestion under normal conditions or take the lead on injury response.

Scientists at the Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA) have now demonstrated that these functions are two sides of a coin: upon injury, one ‘molecular switch’ is enough to propel the stem cells from one state to the other. Their findings, published in the journal Cell Stem Cell, could be instrumental in improving our understanding of gastric pathologies.

Scientists’ interest in adult stem cells resides in their ability to self-renew indefinitely and differentiate into all cell types making up the organ where they reside. That said, increasing evidence suggests that these are not the only functions of adult stem cells — some may also play a key role in the normal functioning of organs, a phenomenon called ‘homeostasis’, while still maintaining their ability to regenerate damaged tissue upon need. Hence, adult stem cells are more complex than we might have thought, and we might only be starting to scratch the surface of their functional diversity and versatility.

A specific adult stem cell population in the stomach body, known as ‘chief cells’, act as ‘reserve stem cells’ under normal physiological conditions, meaning they do not divide but rather secrete enzymes to help with digestion. However, in the event of tissue damage, the cells suddenly switch to a rapidly proliferating state and regenerate the damaged tissue.

“We knew that this behaviour must depend on a switch, and we aimed to unveil the precise mechanism of this switch,” said IMBA Group Leader Bon-Kyoung Koo, the corresponding author of the new study.

Together with collaborators from Vanderbilt University Medical Center in the US and Pohang University of Science and Technology (POSTECH) in South Korea, the IMBA researchers developed a mouse model to trace the effects of stomach tissue injury at a single-cell level. The team was thus able to characterise all the transcribed genes on a single-cell basis (an approach called ‘transcriptome analysis’) and trace the lineage of the cells in the regenerating mouse stomach tissue.

“With this strategy, we identified one molecule, p57, as the potential molecular switch we had been looking for,” said lead author Ji-Hyun Lee, a postdoctoral fellow in the Koo lab at IMBA. “We confirmed that, upon injury, the level of p57 rapidly decreases in the chief cells and that this is followed by a peak of proliferation within the chief cell lineage.”

To test their hypothesis further and strengthen their findings, the team overexpressed p57 in stomach organoids. This allowed them to induce a long-term reserve stem cell state in the organoids and an increased level of enzyme secretion. In other words, the high p57 levels in the organoids made the reserve stem cells behave more like mature, secretory chief cells that do not proliferate.

“Organoids are usually highly proliferative because of the growth factor cocktail that they need in culture; however, once we introduced p57, the organoids suddenly stopped growing,” Lee said. “In a second step, we reduced the expression of p57, and to our delight, the organoids started proliferating again.

“This means that the cells did not lose their stemness due to p57. They merely went into a reserve state, exactly mimicking our in vivo observations.”

Normal mouse stomach epithelium (top) versus epithelium with p57 overexpression (bottom) at day 3 post-injury with high-dose tamoxifen (HDT). The epithelium is stained for chief cells (magenta), neck cells (green), and proliferating cells (white). Image ©Lee/Koo/Cell Stem Cell/IMBA.

The molecule p57 belongs to a larger family of proteins, so-called CDK inhibitors, that inhibit the cell cycle and proliferation. Drugs with this function have been developed to treat some cancers. The team led by Koo and Lee tested the effect of other molecules of this family on stomach organoids to check if their findings are truly specific to p57. Unlike p57, the other molecules did not induce a reversible reserve stem cell state in the organoids but simply caused them to die.

“Our findings show that p57 is a gatekeeper that imposes the reserve stem cell state of the stomach chief cells in homeostasis,” said Koo, confirming that p57 is indeed the molecular switch they had been looking for.

The findings could have an important impact on our understanding of gastric diseases such as spasmolytic polypeptide-expressing metaplasia (SPEM), a chronic condition known to be pre-malignant. Lee said, “Although SPEM is a chronic condition and our experimental set-up only allows us to test the effects of acute injury, we now believe that SPEM is derived from the stomach chief cells. Hence, understanding the activation of these cells ultimately helps us understand the underlying molecular mechanisms of SPEM.”

Top image credit: ©

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