Synthetic nanoparticles used to treat snakebites
US researchers have demonstrated a new approach to treating snakebites, using nanoparticles to bind to venom toxins and prevent the spread of venom through the body.
The standard treatment for snakebites is the intravenous administration of IgG immune molecules that recognise venoms. However, such antivenom therapies must be administered quickly — and by trained healthcare workers — to be effective and are highly specific to particular venoms. With venomous snakebites currently causing more than 100,000 deaths and 400,000 permanent injuries a year, there is an ongoing need for a treatment which can be used in a rural setting and works against the bites of diverse venomous snakes.
Now, Kenneth Shea and colleagues from the University of California, Irvine have engineered nanoparticles that bind to and sequester an array of phospholipases A2 (PLA2) and three-finger toxin (3FTX) molecules found in Elapidae snake venoms. The Elapidae family is a large family of venomous snakes that includes cobras, kraits, tiger snakes, sea snakes, coral snakes and mambas, among other species.
The researchers tested the ability of the nanoparticles to block Naja nigricollis (black-necked spitting cobra) venom in mice that received varying doses of the nanoparticles, injected into the skin. Envenomings by this snake in sub-Saharan Africa inflict serious cutaneous necrosis that may leave permanent tissue damage in the victims. The results were published in the journal PLOS Neglected Tropical Diseases.
In experiments on isolated cells, the nanoparticles were found to sequester a wide range of Elapidae PLA and 3FTX venoms. Moreover, with collaborator José María Gutiérrez from the Universidad de Costa Rica, experiments with mice demonstrated that injections of the nanoparticles at the site of venom injection significantly mitigated the typical necrotic effects — including blistering and ulcers — of the spitting cobra venom. The nanoparticles administered to mice that had not received venom did not have an effect on skin and did not induce systemic toxicity.
“The stable, low-cost nanoparticles have the potential to be administered subcutaneously immediately after the bite at the site of envenoming by this spitting cobra to halt or reduce the extent of local damage and mitigate the systemic distribution of toxins post-envenoming,” the researchers wrote.
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