Bio-Rad Laboratories SEQuoia Complete Stranded RNA Library Prep Kit

Bio-Rad Laboratories has announced the SEQuoia Complete Stranded RNA Library Prep Kit — a novel approach to RNA-Seq library preparation. The high-performance kit offers a holistic view of the complete transcriptome in a simplified workflow and is compatible with a broad range of sample inputs from a variety of sample types, including degraded RNA specimens.

The product overcomes common challenges often experienced with traditional RNA-Seq library preparation kits. Scientists can capture the complete transcriptome, including short (<200 bp) and long (>200 bp) RNAs, in a single library prep. This innovative approach is compatible with a broad range of sample inputs from a variety of sample types, including fresh-frozen, formalin-fixed paraffin-embedded (FFPE) and liquid biopsy specimens, generating cDNA libraries suitable for strand-specific NGS on Illumina sequencers in less than 4 h.

At the core of the kit is SEQzyme enzyme technology, which couples cDNA synthesis with adapter addition in a continuous synthesis reaction, constructing sensitive and diverse libraries compatible with Illumina NGS platforms. The kit also includes access to the integrated SeqSense Analysis Solution. SeqSense is specifically designed for use with the SEQuoia Complete Kit to process and QC sequencing data for all RNA biotypes in the library within a single analysis pipeline.